Search result for:


Cell Line: V79
Cell Type: fibroblast
Description:  Chinese hamster lung
other names: V-79
application: pDNA
Recommendation:  ScreenFect A-plus

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A plus

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,15 µl 0,9 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:2 1:3 1:4
Cell number (cells/well) 30.000-35.000 - -
to achieve 90-100% confluency 48h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 69 % - -
Viability: 92% - -
       
ScreenFect Enhacer addition leads to the following results:      
Transefection efficiency increase: 13% - -

Note: The Two-Step method leads to decreased transfection results.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A


Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 1,8 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:4 1:6 1:6
Cell number (cells/well) 30.000-35.000 - -
to achieve 90-100% confluency 48h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 53 % - -
Viability: 94% - -
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: 19% - -

Note: The Two-Step method leads to decreased transfection results.



ScreenFect_A-Aplus_neu_4.jpg

empty